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     首頁 > 產品展示 > 安捷倫液相色譜儀 > 液相色譜儀 > ELSD 安捷倫G4218
     
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    Waters 2424揮發性光散射偵測器(ELSD)
     
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    產品型號:
    安捷倫380
    產品名稱:
    ELSD 安捷倫G4218
    產品報價:
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      安捷倫380ELSD 安捷倫G4218的詳細資料:

     Agilent 380 ELSD  G4218





     

    Introduction to the ELSD(簡介)

      The Evaporative Light Scattering Detector (Agilent 380/385-ELSD) is a unique

    and highly sensitive detector for semi-volatile and non-volatile solutes in a

    liquid stream. It is mainly used as a concentration detector for High

    Performance Liquid Chromatography (HPLC). The solvent stream containing

    the solute material is nebulized and carried by a gas flow through an

    evaporation chamber. The solvent is volatilized, leaving a mist of solute

    particles that scatter light to a photosensitive device. The signal is amplified

    and a voltage output provides the concentration of the solute particles passing

    through the light.

    The Agilent 380/385-ELSD may be used alone, or as one of several detectors in

    a GPC or HPLC system. As the solvent or eluent is evaporated in the course of

    the analysis, the Agilent 380/385-ELSD must be the last in series if used in

    conjunction with other detectors. If the Agilent 380/385-ELSD is being used as

    the last detector in a series, care must be taken not to exceed the

    recommended backpressure in detector cells in other units.

    This manual instructs the user in the installation and operation of the Agilent

    380/385-ELSD for stand-alone use and control using Agilent OpenLAB

    (ChemStation & EZChrom editions) and Agilent ChemStation.

     

     

    Operational Parameters(操作參數)

     

      The ELS Detector responds to all compounds that are less volatile than the

    mobile phase and is independent of a compound’s optical properties. It

    therefore provides advantages over other spectroscopic detectors for detecting

    compounds that are deficient in a UV chromophore or fluorophore.

    The removal of aqueous mobile phase within an ELSD is typically achieved by

    setting the evaporator temperature to the eluent’s boiling point (e.g. 100 °C) in

    order to remove the solvent. For non-volatile compounds, operating at these

    high temperatures maximizes the signal response.

    However, at these temperatures volatile and semi-volatile compounds are

    destroyed and are therefore not detected. This is problematic for small

    molecules, such as pharmaceuticals and drug candidates.

    The ELS detectors are specifically designed to evaporate difficult solvents at

    ambient and sub-temperatures in order to maximize detection of semi-volatile

    compounds.

    The ELSD has patented technology that reduces the evaporation time of highly

    aqueous solvents at low temperature, and also prevents the evaporation tube

    becoming saturated, which would otherwise preventing further evaporation

    occurring.

    Using this patented evaporation gas technology, a 20 μm droplet of water at

    30 °C can be dried ca. 3x faster than just temperature alone. Using the Agilent

    385-ELSD (G4261A), water can be evaporated as low as 20 °C, providing

    maximum sensitivity to thermally sensitive compounds. For maximum

    sensitivity of non-volatile compounds the evaporation gas can be turned off at

    higher evaporation temperatures.

    Therefore, unlike other ELS detectors, where the evaporator temperature is

    set according to the type of mobile phase, the ELS detector evaporator

    temperature is independent of the mobile phase. Consequently, the ELS

    detector can be set at 30 °C for all types of mobile phase provided the

    evaporator gas flow is adjusted accordingly. This method of operation ensures

    that the ELSD sensitivity is maximized even for low molecular weight

    compounds.

      To prevent against unnecessary gas usage, a controlled gas shut off valve is

    integrated into the detector gas manifold. This will only allow gas to pass into

    the instrument when in RUN mode. Should the instrument default to

    STANDBY mode the gas will reduce to a default value of 1.2 SLM for 15 min

    before closing.

     Parts and Materials(零件與材料)

    Evaporative Light Scattering Detector Parts 蒸發光散射檢測器部分

     



    序號

    說明

    部件號

    1

    Nebulizer Assembly [1]

    see list below

    2

    Nebulization Chamber(glass)[2]

    G4218-40000

    3

    Black Plastic Nut for Nebulization Chamber (13 mm Diameter) [3]

    G4218-40010

    9

    Black Plastic Nut for Nebulization Chamber (30 mm Diameter) [9]

    G4218-40011

    Seal kit for nebulization chambe

    G4218-68010

    Cartridge for Gas Regulator

    G4218-40150

    4、5

    Drain Assembly (includes fitting [5]) [4]

    G4218-40100

    Drain Bottle Assy

    G1946-60111

    ¾ inch PE Tubing for Drain Bottle, 1 unit = 1 cm

    0890-1727

    Tube Fitting (6 mm diameter) for Gas Regulator

    G4218-40160

    6

    Pneumatic tube (diameter 4 mm) for Nebulizer

    (includes stainless steel fitting [6])

    G4218-40220

    Detector Gas Inlet Tube 6 mm O.D., 10 m

    G4218-40170

    7

    Wall Mounting Fitting (4 mm diameter) [7]

    G4218-40140

    8

    Bulkhead Fitting [8]

    G4218-40130

    11

    Gas check valve

    G4218-40200

    Front panel shield window

    G4218-40400

    Autozero Cable

    G4218-81101

    Signal Cable

    G4218-81100

    RS232 Cable Female/Female/Straight, 3 m

    G4218-81103

    External Event Cable

    G4218-81102

    APG Remote Cable

    5061-3378

    Caffeine Standard 250 μg/ml in water

    G4218-85000

     

     

    Nebulizers for the G4218A Agilent Evaporative Light Scattering Detector

    (用于G4218A霧化器)

    Nebulizer

    Useable Flow

    Range

    Optimum

    Flow Range

    Nebulizer Back

    Pressure - bar

    (with water)

    Identifying

    Marks

    Part Number

    Micro Flow

    Nebulizer

    5.0 μl/min –

    40.0 μl/min

    15.0 μl/min –

    25.0 μl/min

    24 (100 μl/min

    Blue Seal

    2 Rings

    G4218-20003

    Semi Micro Flow

    Nebulizer

    0.04 ml/min –

    1.0 ml/min

    0.1 ml/min –

    0.3 ml/min

    44 (1 ml/min)

    Yellow Seal

    2 Rings

    G4218-20001

    Standard Flow

    Nebulizer

    0.2 ml/min –

    2.0 ml/min

    0.5 ml/min –

    1.2 ml/min

    4 (1 ml/min)

    Black Seal

    2 Rings

    G4218-20000

    Large Flow

    Nebulizer

    1.0 ml/min –

    5.0 ml/min

    2.0 ml/min –

    3.0 ml/min

    4 (1 ml/min)

    Red Seal

    1 Ring

    G4218-20002

    Rapid Resolution

    Nebulizer

    0.2 ml/min –

    1.4 ml/min

    0.4 ml/min –

    1.0 ml/min

    14†

    White Seal

    1 Ring

    G4218-20004

     

     

    Identifying Parts and Materials(識別零件與耗材)

    p/n

    Description

    G4260A

    Agilent 380 Evaporative Light Scattering Detector

    G4261A

    Agilent 385 Evaporative Light Scattering Detector

    G4260-60005

    Injection Trigger Cable

    PL0890-0300

    Analogue output cable

    PL0890-0305

    Gas inlet tube (2 m)

    PL0890-0310

    Exhaust hose (PVC-2 m)

    PL0890-0315

    Waste hose (tygon) 7 cm

    PL0890-0320

    Solvent waste container (500 mL)

    PL0890-0325

    RS232 communication cable

    PL0890-0345

    Dimension trigger cable

    PL0890-0350

    Remote start cable (third party LCs only)

    G4260-90000

    ELSD Operation Manual

    PL0890-0370

    ELSD Control Software

    PL0890-0375

    ELSD Dimension Software (includes trigger cable)

    PL0890-0390

    ELSD nebuliser

    PL0890-0395

    Leak sensor assembly

    PL0890-0400

    Mass flow controller

    PL0890-0405

    Photo detector assembly

    PL0890-0410

    ELSD light source

    PL0890-0415

    Fan assembly

    PL0890-0420

    Front (white) LED assembly

    PL0890-0425

    Gas inlet assembly

    PL0890-0430

    AR20 regulator assembly

    PL0890-0435

    Front outlet tube

    PL0890-0440

    ELSD key

    PL0890-0445

    Front panel moulding

    PL0890-0450

    Solvent inlet connector (1/16th)

    PL0890-0455

    ELSD grey keypad

    PL0890-0460

    380-ELSD display window

    PL0890-0465

    385-ELSD display window

    PL0890-0470

    380-ELSD main control PCB

    PL0890-0475

    Evaporator cartridge

    PL0890-0480

    Evaporator heater assembly (no cartridge)

    PL0890-0485

    Neb mount assembly

    PL0890-0490

    Neb chamber assembly

    PL0890-0495

    Exhaust tube

    PL0890-0500

    Exhaust waste tube

    PL0890-0505

    Glass sleeve, neb chamber

    PL0890-0510

    Vapour sensor

    PL0890-0515

    385-ELSD main control PCB

    PL0890-0520

    380-ELSD power supply

    PL0890-0525

    Gas inlet frit, 10 μm

    PL0890-0530

    Gas inlet plug

    PL0890-0535

    385-ELSD power supply

    PL0890-0540

    FFKM O-ring (19.30x2.40)

    PL0890-0545

    FFKM Neb O-Ring (6.0x1.0)

    PL0890-0550

    PTFE nebuliser chamber

    PL0890-0555

    Nebuliser waste tube

    PL0890-0560

    P-Trap diffusor

    PL0890-0565

    Viton O-ring (19.30x2.40)

    PL0890-0570

    Viton O-ring (0.7x1.0)

    PL0890-0575

    Viton O-ring (15.30x2.40)

    PL0890-0580

    Light trap assembly

    PL0890-0585

    Prism assembly

    PL0890-0595

    Optics heater

    PL0890-0615

    O-ring (21.95x1.78)

    PL0890-0620

    O-ring nitrile, prism

    PL0890-0630

    385-ELSD peltier assembly

    PL0890-0635

    Mains inlet fuse (Pk 5)

    8121-1013

    Serial/USB adapter

     

     

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    產品相關關鍵字:ELSD 安捷倫液相 安捷倫蒸發光檢測器 蒸發光檢測器 G4218 380ELSD Agilent  
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